Adjustment of cryoprotectant exposure time to counteract reduced cooling rates
نویسندگان
چکیده
منابع مشابه
Effect of freezing rate and exposure time to cryoprotectant on the development of mouse pronuclear stage embryos.
BACKGROUND The effects of exposure time (20 versus 45 s) to a high concentration of cryoprotectant (7.0 mol/l ethylene glycol with 0.5 mol/l sucrose) and freezing rates (1200-10 300 degrees C/min) during rapid freezing of mouse pronuclear stage embryos on survival and development to blastocysts were investigated. Different freezing rates were achieved by directly plunging the straws (rapid free...
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Many plant species can be cryopreserved by treating shoot tips with complex cryoprotectant solutions before rapidly cooling them to liquid nitrogen temperatures. Plant vitrification solution 2 (PVS2), a commonly selected cryoprotectant, can be lethal with extended exposure times. To determine potentially toxic combinations, we have exposed mint shoot tips to one-, two-, three-, and four-compone...
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The most commonly reported measures of prognosis for patients with cancer or some other chronic disease are 5-or 10-year absolute or relative survival rates. Because survival rates often vary by age of patients, and because the age structure of patients often varies between populations, age adjustment is crucial for comparative analyses of survival rates. However, traditional age adjustment oft...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2012
ISSN: 1472-6483
DOI: 10.1016/j.rbmo.2012.02.018